RapamycinFungusV1, Main, Exploration, bibRecord, 000178

Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.

Identifieur interne : 000178 ( Main/Exploration ); précédent : 000177; suivant : 000179

Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.

Auteurs : Jérôme Savocco [Belgique] ; Sylvain Nootens [Belgique] ; Wilhelmine Afokpa [Belgique] ; Mathilde Bausart [Belgique] ; Xiaoqian Chen [Belgique] ; Jennifer Villers [Belgique] ; Henri-François Renard [Belgique] ; Martine Prévost [Belgique] ; Ruddy Wattiez [Belgique] ; Pierre Morsomme [Belgique]

Source :

PLoS biology [ 1545-7885 ] ; 2019.

RBID : pubmed:31658248

Descripteurs français

English descriptors

Abstract

Endocytosis of membrane proteins in yeast requires α-arrestin-mediated ubiquitylation by the ubiquitin ligase Rsp5. Yet, the diversity of α-arrestin targets studied is restricted to a small subset of plasma membrane (PM) proteins. Here, we performed quantitative proteomics to identify new targets of 12 α-arrestins and gained insight into the diversity of pathways affected by α-arrestins, including the cell wall integrity pathway and PM-endoplasmic reticulum contact sites. We found that Art2 is the main regulator of substrate- and stress-induced ubiquitylation and endocytosis of the thiamine (vitamin B1) transporters: Thi7, nicotinamide riboside transporter 1 (Nrt1), and Thi72. Genetic screening allowed for the isolation of transport-defective Thi7 mutants, which impaired thiamine-induced endocytosis. Coexpression of inactive mutants with wild-type Thi7 revealed that both transporter conformation and transport activity are important to induce endocytosis. Finally, we provide evidence that Art2 mediated Thi7 endocytosis is regulated by the target of rapamycin complex 1 (TORC1) and requires the Sit4 phosphatase but is not inhibited by the Npr1 kinase.

DOI: 10.1371/journal.pbio.3000512
PubMed: 31658248
PubMed Central: PMC6837554

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000166
to stream Main, to step Curation: 000166

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.</title>
<author><name sortKey="Savocco, Jerome" sort="Savocco, Jerome" uniqKey="Savocco J" first="Jérôme" last="Savocco">Jérôme Savocco</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nootens, Sylvain" sort="Nootens, Sylvain" uniqKey="Nootens S" first="Sylvain" last="Nootens">Sylvain Nootens</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Afokpa, Wilhelmine" sort="Afokpa, Wilhelmine" uniqKey="Afokpa W" first="Wilhelmine" last="Afokpa">Wilhelmine Afokpa</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bausart, Mathilde" sort="Bausart, Mathilde" uniqKey="Bausart M" first="Mathilde" last="Bausart">Mathilde Bausart</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Xiaoqian" sort="Chen, Xiaoqian" uniqKey="Chen X" first="Xiaoqian" last="Chen">Xiaoqian Chen</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Villers, Jennifer" sort="Villers, Jennifer" uniqKey="Villers J" first="Jennifer" last="Villers">Jennifer Villers</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Renard, Henri Francois" sort="Renard, Henri Francois" uniqKey="Renard H" first="Henri-François" last="Renard">Henri-François Renard</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Prevost, Martine" sort="Prevost, Martine" uniqKey="Prevost M" first="Martine" last="Prévost">Martine Prévost</name>
<affiliation wicri:level="4"><nlm:affiliation>Structure and Function of Biological Membranes, Université libre de Bruxelles, Brussels, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Structure and Function of Biological Membranes, Université libre de Bruxelles, Brussels</wicri:regionArea>
<placeName><settlement type="city">Bruxelles</settlement>
<region nuts="2">Région de Bruxelles-Capitale</region>
</placeName>
<orgName type="university">Université libre de Bruxelles</orgName>
</affiliation>
</author>
<author><name sortKey="Wattiez, Ruddy" sort="Wattiez, Ruddy" uniqKey="Wattiez R" first="Ruddy" last="Wattiez">Ruddy Wattiez</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Proteomics and Microbiology, Research Institute for Biosciences, Université de Mons, Mons, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Department of Proteomics and Microbiology, Research Institute for Biosciences, Université de Mons, Mons</wicri:regionArea>
<orgName type="university">Université de Mons</orgName>
<placeName><settlement type="city">Liège</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province de Hainaut</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morsomme, Pierre" sort="Morsomme, Pierre" uniqKey="Morsomme P" first="Pierre" last="Morsomme">Pierre Morsomme</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:31658248</idno>
<idno type="pmid">31658248</idno>
<idno type="doi">10.1371/journal.pbio.3000512</idno>
<idno type="pmc">PMC6837554</idno>
<idno type="wicri:Area/Main/Corpus">000166</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000166</idno>
<idno type="wicri:Area/Main/Curation">000166</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000166</idno>
<idno type="wicri:Area/Main/Exploration">000166</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.</title>
<author><name sortKey="Savocco, Jerome" sort="Savocco, Jerome" uniqKey="Savocco J" first="Jérôme" last="Savocco">Jérôme Savocco</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nootens, Sylvain" sort="Nootens, Sylvain" uniqKey="Nootens S" first="Sylvain" last="Nootens">Sylvain Nootens</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Afokpa, Wilhelmine" sort="Afokpa, Wilhelmine" uniqKey="Afokpa W" first="Wilhelmine" last="Afokpa">Wilhelmine Afokpa</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bausart, Mathilde" sort="Bausart, Mathilde" uniqKey="Bausart M" first="Mathilde" last="Bausart">Mathilde Bausart</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Xiaoqian" sort="Chen, Xiaoqian" uniqKey="Chen X" first="Xiaoqian" last="Chen">Xiaoqian Chen</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Villers, Jennifer" sort="Villers, Jennifer" uniqKey="Villers J" first="Jennifer" last="Villers">Jennifer Villers</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Renard, Henri Francois" sort="Renard, Henri Francois" uniqKey="Renard H" first="Henri-François" last="Renard">Henri-François Renard</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Prevost, Martine" sort="Prevost, Martine" uniqKey="Prevost M" first="Martine" last="Prévost">Martine Prévost</name>
<affiliation wicri:level="4"><nlm:affiliation>Structure and Function of Biological Membranes, Université libre de Bruxelles, Brussels, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Structure and Function of Biological Membranes, Université libre de Bruxelles, Brussels</wicri:regionArea>
<placeName><settlement type="city">Bruxelles</settlement>
<region nuts="2">Région de Bruxelles-Capitale</region>
</placeName>
<orgName type="university">Université libre de Bruxelles</orgName>
</affiliation>
</author>
<author><name sortKey="Wattiez, Ruddy" sort="Wattiez, Ruddy" uniqKey="Wattiez R" first="Ruddy" last="Wattiez">Ruddy Wattiez</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Proteomics and Microbiology, Research Institute for Biosciences, Université de Mons, Mons, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Department of Proteomics and Microbiology, Research Institute for Biosciences, Université de Mons, Mons</wicri:regionArea>
<orgName type="university">Université de Mons</orgName>
<placeName><settlement type="city">Liège</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province de Hainaut</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morsomme, Pierre" sort="Morsomme, Pierre" uniqKey="Morsomme P" first="Pierre" last="Morsomme">Pierre Morsomme</name>
<affiliation wicri:level="4"><nlm:affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve</wicri:regionArea>
<orgName type="university">Université catholique de Louvain</orgName>
<placeName><settlement type="city">Louvain-la-Neuve</settlement>
<region type="region" nuts="1">Région wallonne</region>
<region type="province" nuts="1">Province du Brabant wallon</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS biology</title>
<idno type="eISSN">1545-7885</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arrestins (genetics)</term>
<term>Arrestins (metabolism)</term>
<term>Cell Membrane (drug effects)</term>
<term>Cell Membrane (genetics)</term>
<term>Cell Membrane (metabolism)</term>
<term>Cell Wall (drug effects)</term>
<term>Cell Wall (genetics)</term>
<term>Cell Wall (metabolism)</term>
<term>Endocytosis (genetics)</term>
<term>Endoplasmic Reticulum (drug effects)</term>
<term>Endoplasmic Reticulum (genetics)</term>
<term>Endoplasmic Reticulum (metabolism)</term>
<term>Endosomal Sorting Complexes Required for Transport (genetics)</term>
<term>Endosomal Sorting Complexes Required for Transport (metabolism)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Membrane Transport Proteins (genetics)</term>
<term>Membrane Transport Proteins (metabolism)</term>
<term>Models, Molecular (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Nucleoside Transport Proteins (genetics)</term>
<term>Nucleoside Transport Proteins (metabolism)</term>
<term>Protein Binding (MeSH)</term>
<term>Protein Kinases (genetics)</term>
<term>Protein Kinases (metabolism)</term>
<term>Protein Phosphatase 2 (genetics)</term>
<term>Protein Phosphatase 2 (metabolism)</term>
<term>Protein Processing, Post-Translational (MeSH)</term>
<term>Protein Structure, Secondary (MeSH)</term>
<term>Proteomics (methods)</term>
<term>Saccharomyces cerevisiae (drug effects)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
<term>Signal Transduction (MeSH)</term>
<term>Thiamine (metabolism)</term>
<term>Thiamine (pharmacology)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
<term>Ubiquitin-Protein Ligase Complexes (genetics)</term>
<term>Ubiquitin-Protein Ligase Complexes (metabolism)</term>
<term>Ubiquitination (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Arrestines (génétique)</term>
<term>Arrestines (métabolisme)</term>
<term>Complexes de tri endosomique requis pour le transport (génétique)</term>
<term>Complexes de tri endosomique requis pour le transport (métabolisme)</term>
<term>Endocytose (génétique)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Liaison aux protéines (MeSH)</term>
<term>Maturation post-traductionnelle des protéines (MeSH)</term>
<term>Membrane cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Membrane cellulaire (génétique)</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Paroi cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Paroi cellulaire (génétique)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Protein Phosphatase 2 (génétique)</term>
<term>Protein Phosphatase 2 (métabolisme)</term>
<term>Protein kinases (génétique)</term>
<term>Protein kinases (métabolisme)</term>
<term>Protéines de Saccharomyces cerevisiae (génétique)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Protéines de transport membranaire (génétique)</term>
<term>Protéines de transport membranaire (métabolisme)</term>
<term>Protéomique (méthodes)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Réticulum endoplasmique (effets des médicaments et des substances chimiques)</term>
<term>Réticulum endoplasmique (génétique)</term>
<term>Réticulum endoplasmique (métabolisme)</term>
<term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Structure secondaire des protéines (MeSH)</term>
<term>Thiamine (métabolisme)</term>
<term>Thiamine (pharmacologie)</term>
<term>Transduction du signal (MeSH)</term>
<term>Transporteurs de nucléosides (génétique)</term>
<term>Transporteurs de nucléosides (métabolisme)</term>
<term>Ubiquitin-protein ligase complexes (génétique)</term>
<term>Ubiquitin-protein ligase complexes (métabolisme)</term>
<term>Ubiquitination (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arrestins</term>
<term>Endosomal Sorting Complexes Required for Transport</term>
<term>Membrane Transport Proteins</term>
<term>Nucleoside Transport Proteins</term>
<term>Protein Kinases</term>
<term>Protein Phosphatase 2</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
<term>Ubiquitin-Protein Ligase Complexes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arrestins</term>
<term>Endosomal Sorting Complexes Required for Transport</term>
<term>Membrane Transport Proteins</term>
<term>Nucleoside Transport Proteins</term>
<term>Protein Kinases</term>
<term>Protein Phosphatase 2</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Thiamine</term>
<term>Transcription Factors</term>
<term>Ubiquitin-Protein Ligase Complexes</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Membrane</term>
<term>Cell Wall</term>
<term>Endoplasmic Reticulum</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Membrane cellulaire</term>
<term>Paroi cellulaire</term>
<term>Réticulum endoplasmique</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Membrane</term>
<term>Cell Wall</term>
<term>Endocytosis</term>
<term>Endoplasmic Reticulum</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arrestines</term>
<term>Complexes de tri endosomique requis pour le transport</term>
<term>Endocytose</term>
<term>Facteurs de transcription</term>
<term>Membrane cellulaire</term>
<term>Paroi cellulaire</term>
<term>Protein Phosphatase 2</term>
<term>Protein kinases</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de transport membranaire</term>
<term>Réticulum endoplasmique</term>
<term>Saccharomyces cerevisiae</term>
<term>Transporteurs de nucléosides</term>
<term>Ubiquitin-protein ligase complexes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Cell Wall</term>
<term>Endoplasmic Reticulum</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arrestines</term>
<term>Complexes de tri endosomique requis pour le transport</term>
<term>Facteurs de transcription</term>
<term>Membrane cellulaire</term>
<term>Paroi cellulaire</term>
<term>Protein Phosphatase 2</term>
<term>Protein kinases</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de transport membranaire</term>
<term>Réticulum endoplasmique</term>
<term>Saccharomyces cerevisiae</term>
<term>Thiamine</term>
<term>Transporteurs de nucléosides</term>
<term>Ubiquitin-protein ligase complexes</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Thiamine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Models, Molecular</term>
<term>Mutation</term>
<term>Protein Binding</term>
<term>Protein Processing, Post-Translational</term>
<term>Protein Structure, Secondary</term>
<term>Signal Transduction</term>
<term>Ubiquitination</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Liaison aux protéines</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Modèles moléculaires</term>
<term>Mutation</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Structure secondaire des protéines</term>
<term>Transduction du signal</term>
<term>Ubiquitination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Endocytosis of membrane proteins in yeast requires α-arrestin-mediated ubiquitylation by the ubiquitin ligase Rsp5. Yet, the diversity of α-arrestin targets studied is restricted to a small subset of plasma membrane (PM) proteins. Here, we performed quantitative proteomics to identify new targets of 12 α-arrestins and gained insight into the diversity of pathways affected by α-arrestins, including the cell wall integrity pathway and PM-endoplasmic reticulum contact sites. We found that Art2 is the main regulator of substrate- and stress-induced ubiquitylation and endocytosis of the thiamine (vitamin B1) transporters: Thi7, nicotinamide riboside transporter 1 (Nrt1), and Thi72. Genetic screening allowed for the isolation of transport-defective Thi7 mutants, which impaired thiamine-induced endocytosis. Coexpression of inactive mutants with wild-type Thi7 revealed that both transporter conformation and transport activity are important to induce endocytosis. Finally, we provide evidence that Art2 mediated Thi7 endocytosis is regulated by the target of rapamycin complex 1 (TORC1) and requires the Sit4 phosphatase but is not inhibited by the Npr1 kinase.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31658248</PMID>
<DateCompleted><Year>2020</Year>
<Month>02</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>02</Month>
<Day>27</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1545-7885</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>17</Volume>
<Issue>10</Issue>
<PubDate><Year>2019</Year>
<Month>10</Month>
</PubDate>
</JournalIssue>
<Title>PLoS biology</Title>
<ISOAbbreviation>PLoS Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.</ArticleTitle>
<Pagination><MedlinePgn>e3000512</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pbio.3000512</ELocationID>
<Abstract><AbstractText>Endocytosis of membrane proteins in yeast requires α-arrestin-mediated ubiquitylation by the ubiquitin ligase Rsp5. Yet, the diversity of α-arrestin targets studied is restricted to a small subset of plasma membrane (PM) proteins. Here, we performed quantitative proteomics to identify new targets of 12 α-arrestins and gained insight into the diversity of pathways affected by α-arrestins, including the cell wall integrity pathway and PM-endoplasmic reticulum contact sites. We found that Art2 is the main regulator of substrate- and stress-induced ubiquitylation and endocytosis of the thiamine (vitamin B1) transporters: Thi7, nicotinamide riboside transporter 1 (Nrt1), and Thi72. Genetic screening allowed for the isolation of transport-defective Thi7 mutants, which impaired thiamine-induced endocytosis. Coexpression of inactive mutants with wild-type Thi7 revealed that both transporter conformation and transport activity are important to induce endocytosis. Finally, we provide evidence that Art2 mediated Thi7 endocytosis is regulated by the target of rapamycin complex 1 (TORC1) and requires the Sit4 phosphatase but is not inhibited by the Npr1 kinase.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Savocco</LastName>
<ForeName>Jérôme</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0003-0989-9473</Identifier>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nootens</LastName>
<ForeName>Sylvain</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Afokpa</LastName>
<ForeName>Wilhelmine</ForeName>
<Initials>W</Initials>
<Identifier Source="ORCID">0000-0002-9177-7485</Identifier>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bausart</LastName>
<ForeName>Mathilde</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Xiaoqian</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Villers</LastName>
<ForeName>Jennifer</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0003-3832-3481</Identifier>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Renard</LastName>
<ForeName>Henri-François</ForeName>
<Initials>HF</Initials>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Prévost</LastName>
<ForeName>Martine</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Structure and Function of Biological Membranes, Université libre de Bruxelles, Brussels, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wattiez</LastName>
<ForeName>Ruddy</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Proteomics and Microbiology, Research Institute for Biosciences, Université de Mons, Mons, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Morsomme</LastName>
<ForeName>Pierre</ForeName>
<Initials>P</Initials>
<Identifier Source="ORCID">0000-0001-7780-7230</Identifier>
<AffiliationInfo><Affiliation>Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>figshare</DataBankName>
<AccessionNumberList><AccessionNumber>10.6084/m9.figshare.9924656</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>10</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS Biol</MedlineTA>
<NlmUniqueID>101183755</NlmUniqueID>
<ISSNLinking>1544-9173</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019390">Arrestins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D056827">Endosomal Sorting Complexes Required for Transport</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D026901">Membrane Transport Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C528866">Nrt1 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D033703">Nucleoside Transport Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C561842">TORC1 protein complex, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C000592466">Thi7 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C000592467">Thi72 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147682-31-3</RegistryNumber>
<NameOfSubstance UI="C066958">NPR1 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.3.2.23</RegistryNumber>
<NameOfSubstance UI="D043743">Ubiquitin-Protein Ligase Complexes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.-</RegistryNumber>
<NameOfSubstance UI="D011494">Protein Kinases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.3.16</RegistryNumber>
<NameOfSubstance UI="D054648">Protein Phosphatase 2</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.3.16</RegistryNumber>
<NameOfSubstance UI="C104343">SIT4 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 6.3.2.-</RegistryNumber>
<NameOfSubstance UI="C469287">RSP5 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>X66NSO3N35</RegistryNumber>
<NameOfSubstance UI="D013831">Thiamine</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D019390" MajorTopicYN="N">Arrestins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004705" MajorTopicYN="N">Endocytosis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004721" MajorTopicYN="N">Endoplasmic Reticulum</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056827" MajorTopicYN="N">Endosomal Sorting Complexes Required for Transport</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D026901" MajorTopicYN="N">Membrane Transport Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D033703" MajorTopicYN="N">Nucleoside Transport Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011494" MajorTopicYN="N">Protein Kinases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054648" MajorTopicYN="N">Protein Phosphatase 2</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011499" MajorTopicYN="Y">Protein Processing, Post-Translational</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017433" MajorTopicYN="N">Protein Structure, Secondary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013831" MajorTopicYN="N">Thiamine</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D043743" MajorTopicYN="N">Ubiquitin-Protein Ligase Complexes</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054875" MajorTopicYN="N">Ubiquitination</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<CoiStatement>The authors have declared that no competing interests exist.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>04</Month>
<Day>24</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>10</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>11</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>10</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>10</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31658248</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pbio.3000512</ArticleId>
<ArticleId IdType="pii">PBIOLOGY-D-19-01147</ArticleId>
<ArticleId IdType="pmc">PMC6837554</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Mol Cell Biol. 2012 Nov;32(22):4510-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22966204</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 1991 Apr 2;1063(2):265-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1826456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2009 Aug 15;23(16):1929-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19684113</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Jan 08;9(1):e85418</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24416406</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Elife. 2018 Mar 23;7:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29570051</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2013 Aug 16;288(33):24063-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23824189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2014 Aug 8;289(32):22103-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24942738</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Evol Biol. 2008 Jul 29;8:222</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18664266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2012 Jan 23;196(2):247-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22249293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2017 Oct 15;28(21):2819-2832</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28814503</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2018 Aug 15;29(17):2113-2127</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29927345</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 2010 Feb 1;78(2):365-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19722269</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2010 Feb;30(4):897-907</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20028738</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2015 Mar;35(6):939-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25547292</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Sci. 2015 Nov 15;128(22):4220-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26459639</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2019 Jan 25;20(3):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30691068</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2011 Nov 23;147(5):1104-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22118465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2013 Jun 7;288(23):16206-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23645667</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Chem. 2004 Mar 25;47(7):1739-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15027865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Elife. 2014 Nov 07;3:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25380227</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2014;5:3101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24476960</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eukaryot Cell. 2012 Apr;11(4):463-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22307975</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2017 Nov 24;429(23):3678-3695</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28965784</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Traffic. 2009 Dec;10(12):1856-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19912579</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genomics. 2009 Apr;10(2):133-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19794886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2015 Jun 1;26(11):2128-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25851600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eukaryot Cell. 2014 Nov;13(11):1380-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25172766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2002 Aug 16;277(33):29608-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12048206</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2012 Jun 28;9(7):676-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22743772</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1993 Aug 15;268(23):17440-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8394343</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2014 Jul;34(14):2660-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24820415</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2008 Oct 31;322(5902):709-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18927357</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2010 Apr 23;328(5977):470-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20413494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Chem Biol. 2013 Sep;9(9):565-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831759</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2017 Jun 5;216(6):1811-1831</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28468835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2016 Dec 15;27(25):4043-4054</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27798240</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2011 Nov;10(11):M111.009589</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21825281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 2003;374:461-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14696385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Res Int. 2012;2012:242764</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22988512</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1998 Dec 1;17(23):6924-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9843498</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2008 Nov 14;135(4):714-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18976803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2018 Jul 13;293(28):10926-10936</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29789424</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2017 Sep;16(9):1652-1668</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28679684</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2012;8(11):e1003083</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23209439</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Can J Microbiol. 2005 Oct;51(10):833-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16333342</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2016 May;203(1):299-317</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26920760</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1995 Nov;15(11):5879-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7565740</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1998 Jan;18(1):314-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9418878</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Genet Genomics. 2006 Aug;276(2):147-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16850348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 1995 Oct;18(1):77-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8596462</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1985 Oct;150(1):76-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3843705</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2015 Feb 6;14(2):738-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25495031</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Evol Biol. 2005 Sep;18(5):1368-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16135132</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 Apr 1;23(7):802-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17267437</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 1994 Apr;10(4):481-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7941734</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 1998 Jun;9(6):1253-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9614172</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3145-E3154</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29559531</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1996 May 3;271(18):10946-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8631913</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1988;157:528-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2906716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1996 Jan 26;84(2):277-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8565073</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2017 May 1;28(9):1271-1283</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28298493</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2014 Dec;34(24):4447-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25266656</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2001 May 14;153(4):649-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11352928</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2009 Jun 29;185(7):1227-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19564405</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2014 Mar 15;30(6):884-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24162465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Traffic. 2013 Apr;14(4):412-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23305501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2014 Aug 18;33(16):1831-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24952894</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2010 Oct 15;21(20):3552-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20739461</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1995 Feb 10;270(6):2525-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7852314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2014 Aug;13(8):1979-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24961812</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2009 May;6(5):343-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19363495</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2000 Aug 4;275(31):23608-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10811641</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
</country>
<region><li>Province de Hainaut</li>
<li>Province du Brabant wallon</li>
<li>Région de Bruxelles-Capitale</li>
<li>Région wallonne</li>
</region>
<settlement><li>Bruxelles</li>
<li>Liège</li>
<li>Louvain-la-Neuve</li>
</settlement>
<orgName><li>Université catholique de Louvain</li>
<li>Université de Mons</li>
<li>Université libre de Bruxelles</li>
</orgName>
</list>
<tree><country name="Belgique"><region name="Région wallonne"><name sortKey="Savocco, Jerome" sort="Savocco, Jerome" uniqKey="Savocco J" first="Jérôme" last="Savocco">Jérôme Savocco</name>
</region>
<name sortKey="Afokpa, Wilhelmine" sort="Afokpa, Wilhelmine" uniqKey="Afokpa W" first="Wilhelmine" last="Afokpa">Wilhelmine Afokpa</name>
<name sortKey="Bausart, Mathilde" sort="Bausart, Mathilde" uniqKey="Bausart M" first="Mathilde" last="Bausart">Mathilde Bausart</name>
<name sortKey="Chen, Xiaoqian" sort="Chen, Xiaoqian" uniqKey="Chen X" first="Xiaoqian" last="Chen">Xiaoqian Chen</name>
<name sortKey="Morsomme, Pierre" sort="Morsomme, Pierre" uniqKey="Morsomme P" first="Pierre" last="Morsomme">Pierre Morsomme</name>
<name sortKey="Nootens, Sylvain" sort="Nootens, Sylvain" uniqKey="Nootens S" first="Sylvain" last="Nootens">Sylvain Nootens</name>
<name sortKey="Prevost, Martine" sort="Prevost, Martine" uniqKey="Prevost M" first="Martine" last="Prévost">Martine Prévost</name>
<name sortKey="Renard, Henri Francois" sort="Renard, Henri Francois" uniqKey="Renard H" first="Henri-François" last="Renard">Henri-François Renard</name>
<name sortKey="Villers, Jennifer" sort="Villers, Jennifer" uniqKey="Villers J" first="Jennifer" last="Villers">Jennifer Villers</name>
<name sortKey="Wattiez, Ruddy" sort="Wattiez, Ruddy" uniqKey="Wattiez R" first="Ruddy" last="Wattiez">Ruddy Wattiez</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000178 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000178 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    RapamycinFungusV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:31658248
   |texte=   Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:31658248" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a RapamycinFungusV1

This area was generated with Dilib version V0.6.38.
Data generation: Thu Nov 19 21:55:41 2020. Site generation: Thu Nov 19 22:00:39 2020

	Serveur d'exploration sur la rapamycine et les champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la rapamycine et les champignons

Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.

Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki